The Complete Amino Acid Sequence of Chitinase - a from the Seeds of Rye ( Slecale cereat
نویسنده
چکیده
The complete amine acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleayed with cyanogen bromide and the resulting three fragments, CBI, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CBI were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin ef reduced SLcarboxymethylated or S-aminoethylated CBI. The sequences of fragments CB2 and CB3 were established by sequencing the tryptic peptides from reduced S-carboxymethylated CB2 and CB3, and by aligning them with the sequence
منابع مشابه
The complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereal).
The complete amino acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleaved with cyanogen bromide and the resulting three fragments, CB1, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CB1 were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin of reduced S-carboxymethylated ...
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